2,43
1,65
1,98
0,73
1,88
1,81
2,43
2.2 Staðluð efni sem notuð eru í kvörðunarferli hlutfallslegrar sameindamassadreifingar: insúlín, mýkópeptíð, glýsín-glýsín-týrósín-arginín, glýsín-glýsín-glýsín
3 Tæki og búnaður
23.2
21.4
22.2
16.1
22.3
20,8
23,9
27,5
Í heildina er hlutfall amínósýra í vörum Sustar hærra en í vörum Zinpro.
8. hluti Áhrif notkunar
Áhrif mismunandi uppspretta snefilefna á framleiðslugetu og eggjagæði varphæna seint á varptímabilinu
Framleiðsluferli
Markviss kelunartækni
Skerfleytitækni
Þrýstiúða- og þurrkunartækni
Kæli- og rakatækni
Háþróuð umhverfisstýringartækni
Viðauki A: Aðferðir til að ákvarða hlutfallslega sameindamassadreifingu peptíða
Samþykkt staðals: GB/T 22492-2008
1 Prófunarregla:
Það var ákvarðað með háafköstum gel síunarskiljun. Það er að segja, með því að nota porous fylliefni sem kyrrstæðan fasa, byggt á mismun á hlutfallslegri mólmassastærð sýnisþáttanna til aðskilnaðar, greindur við peptíðtengi með útfjólubláu frásogsbylgjulengd 220 nm, með því að nota sérstakan gagnavinnsluhugbúnað til að ákvarða hlutfallslegan mólmassadreifingu með gel síunarskiljun (þ.e. GPC hugbúnaður), voru skiljunarritin og gögn þeirra unnin, reiknuð til að fá stærð hlutfallslegs mólmassa sojabaunapeptíðsins og dreifingarsviðið.
2. Hvarfefni
Tilraunavatnið ætti að uppfylla forskriftir GB/T6682 um aukavatn, notkun hvarfefna, að undanskildum sérstökum ákvæðum, er greiningarhrein.
2.1 Hvarfefni eru meðal annars asetónítríl (skiljunarfræðilega hreint), tríflúorediksýra (skiljunarfræðilega hreint),
2.2 Staðluð efni sem notuð eru í kvörðunarferli hlutfallslegrar sameindamassadreifingar: insúlín, mýkópeptíð, glýsín-glýsín-týrósín-arginín, glýsín-glýsín-glýsín
3 Tæki og búnaður
3.1 Háafkastavökvaskiljunartækni (HPLC): Kiljunarvinnslustöð eða samþættingartæki með útfjólubláum ljósnema og hugbúnaði fyrir gagnavinnslu með GPC.
3.2 Færanleg síunar- og afgasunareining með lofttæmingu.
3.3 Rafræn vog: kvarðað gildi 0,000 1 g.
4 skref í notkun
4.1 Litskiljunarskilyrði og tilraunir með kerfisaðlögun (viðmiðunarskilyrði)
- 4.1.1 Litskiljunarsúla: TSKgelG2000swxl300 mm × 7,8 mm (innra þvermál) eða aðrar gel-súlur af sömu gerð með svipaða afköst sem henta til að ákvarða prótein og peptíð.
- 4.1.2 Færanlegt efni: Asetónítríl + vatn + tríflúorediksýra = 20 + 80 + 0,1.
- 4.1.3 Skynjunarbylgjulengd: 220 nm.
- 4.1.4 Rennslishraði: 0,5 ml/mín.
- 4.1.5 Greiningartími: 30 mín.
- 4.1.6 Rúmmál sýnissprautunar: 20 μL.
- 4.1.7 Hitastig súlunnar: stofuhitastig.
- 4.1.8 Til að litskiljunarkerfið uppfylli kröfur um greiningu var kveðið á um að við ofangreindar litskiljunaraðstæður væri skilvirkni gellitskiljunarsúlunnar, þ.e. fræðilegur fjöldi platna (N), ekki minni en 10000, reiknað út frá tindum þrípeptíðstaðalsins (glýsín-glýsín-glýsín).
- 4.2 Framleiðsla staðalferla fyrir hlutfallslegan mólmassa
- Ofangreindar mismunandi staðlaðar peptíðlausnir með hlutfallslegri mólmassa og massaþéttni 1 mg/ml voru útbúnar með hreyfanlegum fasajöfnun, blandaðar saman í ákveðnu hlutfalli og síðan síaðar í gegnum lífrænan fasahimnu með gatastærð 0,2 μm~0,5 μm og sprautað inn í sýnið, og síðan voru litróf staðlanna fengin. Kvörðunarferlar fyrir hlutfallslegan mólmassa og jöfnur þeirra voru fengnar með því að teikna lógaritma hlutfallslegs mólmassa á móti varðveislutíma eða með línulegri aðhvarfsgreiningu.
4.3 Sýnishornsmeðferð
Vigtið nákvæmlega 10 mg af sýni í 10 ml mælikolbu, bætið smávegis af ferðafasa út í, hristið með ómskoðun í 10 mínútur, þannig að sýnið leysist alveg upp og blandist, þynnið með ferðafasanum upp að voginni og síið síðan í gegnum himnu lífræns fasa með gatastærð 0,2 μm~0,5 μm, og síuvökvinn greindur samkvæmt litskiljunarskilyrðum í A.4.1.
- 5. Útreikningur á hlutfallslegri dreifingu sameindamassa
- Eftir að sýnislausnin sem útbúin var í 4.3 hefur verið greind við litskiljunarskilyrðin í 4.1, er hægt að fá hlutfallslegan mólmassa sýnisins og dreifingarsvið þess með því að setja litskiljunargögn sýnisins inn í kvörðunarferilinn 4.2 með GPC gagnavinnsluhugbúnaði. Dreifingu hlutfallslegra mólmassa mismunandi peptíða er hægt að reikna út með aðferðinni „toppflatarmálsstaðlun“ samkvæmt formúlunni: X=A/A samtals×100
- Í formúlunni: X - Massahlutfall hlutfallslegs mólmassa peptíðs í heildarpeptíðinu í sýninu, %;
- A - Hámarksflatarmál peptíðs með hlutfallslegan mólmassa;
- Heildar A - summa tindflatarmáls hvers peptíðs með hlutfallslegan mólmassa, reiknuð með einum aukastaf.
- 6 Endurtekningarhæfni
- Algildismunurinn á milli tveggja óháðra ákvarðana sem fengnar eru við endurtekningarhæf skilyrði skal ekki vera meiri en 15% af meðaltali ákvarðananna tveggja.
- Viðauki B: Aðferðir til að ákvarða fríar amínósýrur
- Innleiðing staðals: Q/320205 KAVN05-2016
- 1.2 Hvarfefni og efni
- Ísediksýra: greiningarhrein
- Perklórsýra: 0,0500 mól/L
- Vísir: 0,1% kristalfjólublár vísir (ísediki)
- 2. Ákvörðun á fríum amínósýrum
Sýnin voru þurrkuð við 80°C í 1 klukkustund.
Setjið sýnið í þurrt ílát til að kólna náttúrulega niður í stofuhita eða niður í nothæft hitastig.Vigtið um það bil 0,1 g af sýni (nákvæmni 0,001 g) í 250 ml þurra keiluflösku.Farið fljótt yfir í næsta skref til að koma í veg fyrir að sýnið drekki í sig raka úr umhverfinu.Bætið 25 ml af ísediki út í og blandið vel saman í ekki meira en 5 mínútur.Bætið við 2 dropum af kristalfjólubláum vísiTítrið með 0,0500 mól/l (±0,001) staðlaðri títrunarlausn af perklórsýru þar til lausnin breytist úr fjólubláum í endapunkt.
Skráðu magn staðallausnarinnar sem neytt var.
- Framkvæmið núllprófið á sama tíma.
- 3. Útreikningur og niðurstöður
- Magn frírra amínósýru X í hvarfefninu er gefið upp sem massahlutfall (%) og er reiknað samkvæmt formúlunni: X = C × (V1-V0) × 0,1445/M × 100%, í formúlunni:
- C - Styrkur staðlaðrar perklórsýrulausnar í mólum á lítra (mól/L)
- V1 - Rúmmál notað til títrunar sýna með hefðbundinni perklórsýrulausn, í millilítrum (ml).
- Vo - Rúmmál notað fyrir títrunarblank með staðlaðri perklórsýrulausn, í millilítrum (ml);
M - Massi sýnisins, í grömmum (g).
| 0,1445: Meðalmassi amínósýra sem jafngildir 1,00 ml af staðlaðri perklórsýrulausn [c(HClO4) = 1,000 mól/L]. | 4.2.3 Staðlað títrunarlausn fyrir seríumsúlfat: styrkur c [Ce (SO4) 2] = 0,1 mól/L, útbúin samkvæmt GB/T601. | |
| Innleiðing staðla: Q/70920556 71-2024 | 1. Ákvörðunarregla (Fe sem dæmi) | Amínósýrujárnfléttur hafa mjög litla leysni í vatnsfríu etanóli og frjálsar málmjónir eru leysanlegar í vatnsfríu etanóli. Mismunurinn á leysni þessara tveggja í vatnsfríu etanóli var notaður til að ákvarða klóbindingarhraða amínósýrujárnfléttna. |
| Í formúlunni: V1 - rúmmál staðallausnar seríumsúlfats sem notað er til títrunar á prófunarlausninni, ml; | Vatnsfrítt etanól; restin er sú sama og í ákvæði 4.5.2 í GB/T 27983-2011. | 3. Greiningarskref |
| Gerið tvær tilraunir samtímis. Vigtið 0,1 g af sýninu, þurrkað við 103 ± 2 ℃ í 1 klukkustund, með nákvæmni upp á 0,0001 g, bætið 100 ml af vatnsfríu etanóli út í til að leysa það upp, síið, síið leifarnar og þvegið þær með 100 ml af vatnsfríu etanóli að minnsta kosti þrisvar sinnum, flytjið síðan leifarnar í 250 ml keiluflösku, bætið 10 ml af brennisteinssýrulausn út í samkvæmt grein 4.5.3 í GB/T27983-2011, og framkvæmið síðan eftirfarandi skref samkvæmt grein 4.5.3 „Hitið til að leysast upp og látið kólna“ í GB/T27983-2011. Framkvæmið núllprófið á sama tíma. | 4. Ákvörðun á heildarjárninnihaldi | 4.1 Ákvörðunarreglan er sú sama og í ákvæði 4.4.1 í GB/T 21996-2008. |
4.2. Hvarfefni og lausnir
| 4.2.1 Blandað sýra: Bætið 150 ml af brennisteinssýru og 150 ml af fosfórsýru út í 700 ml af vatni og blandið vel saman. | 4.2.2 Vísirlausn natríumdífenýlamínsúlfónat: 5 g/L, útbúin samkvæmt GB/T603. | 4.2.3 Staðlað títrunarlausn fyrir seríumsúlfat: styrkur c [Ce (SO4) 2] = 0,1 mól/L, útbúin samkvæmt GB/T601. | |
| 4.3 Greiningarskref | Gerið tvær tilraunir samtímis. Vigtið 0,1 g af sýni, með nákvæmni 020001 g, setjið í 250 ml keiluflösku, bætið 10 ml af blönduðu sýru út í, eftir upplausn bætið við 30 ml af vatni og 4 dropum af natríum díanilínsúlfónatvísislausn og framkvæmið síðan eftirfarandi skref samkvæmt grein 4.4.2 í GB/T21996-2008. Framkvæmið núllprófið á sama tíma. | 4.4 Framsetning niðurstaðna | Heildarjárninnihald X1 í amínósýrujárnfléttunum, miðað við massahlutfall járns, gildið gefið upp í %, var reiknað samkvæmt formúlu (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - staðallausn seríumsúlfats sem notuð er til títrunar á núlllausn, ml; | V0 - staðallausn seríumsúlfats sem notuð er til títrunar á núlllausn, ml; | C - Raunverulegur styrkur staðallausnar seríumsúlfats, mól/L5. Útreikningur á járninnihaldi í klötumJárninnihald X2 í klóatinu, miðað við massahlutfall járns, gildið gefið upp í %, var reiknað samkvæmt formúlunni: x2 = ((V1-V2) × C × 0,05585)/m1 × 100 |
| Í formúlunni: V1 - rúmmál staðallausnar seríumsúlfats sem notað er til títrunar á prófunarlausninni, ml; | V2 - staðallausn seríumsúlfats sem notuð er til títrunar á núlllausn, ml;nom1 - Massi sýnisins, g. Takið meðaltal samsíða ákvörðunarniðurstaðnanna sem ákvörðunarniðurstöður og algildur mismunur samsíða ákvörðunarniðurstaðnanna er ekki meiri en 0,3%. | 0,05585 - massi járns, gefið upp í grömmum, sem jafngildir 1,00 ml af staðlaðri seríumsúlfatlausn C[Ce(SO4)2.4H20] = 1,000 mól/L.nom1 - Massi sýnisins, g. Takið meðaltal samsíða ákvörðunarniðurstaðnanna sem ákvörðunarniðurstöður og algildur mismunur samsíða ákvörðunarniðurstaðnanna er ekki meiri en 0,3%. | 6. Útreikningur á kelunarhraðaKelbindingarhraði X3, gildið gefið upp í %, X3 = X2/X1 × 100Viðauki C: Aðferðir til að ákvarða klóbindingarhraða Zinpro |
Innleiðing staðals: Q/320205 KAVNO7-2016
1. Hvarfefni og efni
a) Ísedik: greiningarhreint; b) Perklórsýra: 0,0500 mól/l; c) Vísir: 0,1% kristalfjólublár vísir (ísedik)
2. Ákvörðun á fríum amínósýrum
2.1 Sýnin voru þurrkuð við 80°C í 1 klukkustund.
2.2 Setjið sýnið í þurrt ílát til að kólna náttúrulega niður í stofuhita eða niður í nothæft hitastig.
2.3 Vigtið um það bil 0,1 g af sýni (nákvæmt upp á 0,001 g) í 250 ml þurra keilulaga flösku.
2.4 Farið fljótt yfir í næsta skref til að koma í veg fyrir að sýnið drekki í sig raka úr umhverfinu.
2.5 Bætið 25 ml af ísediki út í og blandið vel í ekki meira en 5 mínútur.
2.6 Bætið við 2 dropum af kristalfjólubláum vísi.
2.7 Títrið með 0,0500 mól/l (±0,001) staðlaðri títrunarlausn af perklórsýru þar til lausnin breytist úr fjólubláu í grænt í 15 sekúndur án þess að breyta um lit sem endapunktur.
2.8 Skráið magn staðallausnarinnar sem neytt var.
2.9 Framkvæmið núllprófið á sama tíma.
- 3. Útreikningur og niðurstöður
- Katalónska
- Physicochemical parameters
V1 - Rúmmál notað til títrunar sýna með hefðbundinni perklórsýrulausn, í millilítrum (ml).
Vo - Rúmmál notað fyrir títrunarblank með staðlaðri perklórsýrulausn, í millilítrum (ml);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Heimilisfang: Qingpu-vegur nr. 147, Shouan-bærinn, Pujiang-sýsla, Chengdu-borg, Sichuan-héraði, Kína
Sími: 86-18880477902
Vörur
Ólífræn snefilefni
- Lífræn snefilefni
- svahílí
- Sérsniðin þjónusta
- Flýtileiðir
Fyrirtækjaupplýsingar
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gújaratíska | Smelltu fyrir fyrirspurn | © Höfundarréttur - 2010-2025: Allur réttur áskilinn. | Veftré VINSÆL LEIT Sími |
| Sími | 86-18880477902 | Javanska | Netfang |
| 8618880477902 | kínverska | Franska | |
| Bird | kínverska | Franska | Þýska Spænska |
| Aquatic animals | Japanska | Kóreska | arabíska gríska |
| Tyrkneska | Ítalska | ||
| Ruminant animal g/head day | January 0.75 | Indónesíska Afríkanska Sænska |
Pólska
- Baskneska
- Katalónska
- Physicochemical parameters
Hindí
Laó
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Búlgarska
- Sebúanó
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Króatíska
hollenska
| Application object | Úrdú Víetnamska | Content in full-value feed (mg/kg) | Efficacy |
| Gújaratíska | Haítískt | Hausa | Kínjarvanda Hmong Ungverska |
| Piglets and fattening pigs | Ígbó | Javanska | Kannada Khmer Kúrdísk |
| Kirgisíska | Latína | ||
| Bird | 300～400 | 45～60 | Makedónska Malajíska Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
norska
- Pastú
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbneska
Sesótó
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhí
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
svahílí
Tadsjíka
Tamílska
telúgú
Taílenska
| Application object | Úrdú Víetnamska | Content in full-value feed (mg/kg) | Efficacy |
| jiddíska | Jórúba | Súlú | Kínjarvanda Óría Túrkmenska |
| Úígúrska | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1,52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
